Jul 11, 2014 · ... self-splicing introns in Escherichia coli: the role of the P1 extension helix of the Tetrahymena intron. RNA 8: 647–658. [PMC free article] ...
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Dec 20, 2021 · Here, we demonstrate the activity of engineered hairpin ribozyme variants, which as self-splicing introns are removed from their parent RNA. In ...
Feb 1, 2023 · Fig. 1: Fluorescence splicing assay enables convenient measurement of ribozyme splicing activity. ... a Splicing assay utilizes a plasmid ...
In some cases, they are built into RNA molecules and act upon themselves, as in the self-splicing introns. In other cases, ribozymes act on other RNA molecules, ...
Self-splicing edit. Self-splicing occurs for rare introns that form a ribozyme, performing the functions of the spliceosome by RNA alone. There are three ...
Mar 16, 2023 · Abstract. The group I self-splicing introns, like other RNAs and RNA–protein complexes, undergo multiple conformational changes in completing ...
Jun 5, 2013 · This Tetrahymena intron has been thoroughly investigated and self-splicing was shown to occur by RNA catalysis based on a two-step ...
Group I self-splicing intron; Group II self-splicing intron – Spliceosome is ... "Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA ...
Abstract. The identical reaction pathway executed by the spliceosome and self-splicing group II intron ribozymes has prompted the idea that both may be ...
The term ribozyme was first used by Cech and his colleagues in 1982 to describe the self-splicing ... self-splicing activity. Heterogeneous cleavage was ...